SufB is a composite subunit of iron-sulfur cluster scaffold. Iron-sulfur cluster may play an importa

Suf B

sufB is an iron-sulfur cluster scaffolding complex subunit, and iron-sulfur clusters may play an important role in bacterial salinity adaptation.

sufB is an iron-sulfur cluster scaffolding complex subunit, and iron-sulfur clusters are widely found in various organisms and are involved in electron transfer, substrate binding and activation, gene expression and regulation, etc. This gene may be used to improve saline and alkali tolerance in a variety of microorganisms, especially in model organisms.

In order to verify that the target gene functions in the plant, the plasmid was constructed.

The selected vector is the sequence of E. coli expression vector pHT01 plasmid, and the recombinant plasmid was constructed with 5'Noc I and 3'Hind III as the digestion site.

The reaction system was at 37°C, and the digest was processed for three hours, and then the digest results were detected by agarose gel electrophoresis (1% agarose gel (containing 0.5 μg/mL of nucleic acid dye), 90 V electrophoresis for 30 minutes).

Lane Description( from left to right)

Lane 1: undigested

Lane 2: digested with NcoI and HindIII

Lane 3: ladder（10000bp)

Observing the results of enzyme digestion, it was found that the enzyme bands corresponded to the length of the target gene, which indicated that the target gene was successfully constructed into the expression vector pET28a, and could be transformed in the following steps.

Plasmid transformation: preparation of receptor cells, introduction of plasmids into the receptor cells, and introduction of plasmids into the receptor cells.